Mouse Anti-RNF43 Recombinant Antibody (TAB-327CT)
CAT#: TAB-327CT
This product is a recombinant mouse antibody that recognizes RNF43. The antibody was purified by affinity chromatography. It can be used for WB, ELISA, FC, IHC, IP and other applications.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Anti-Human RNF43 Recombinant Antibody (TAB-327CT) in SDS-PAGE
Lane 1: Non-reduced
Lane 2: Reduced
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Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_7_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108375-RNF43
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Specifications
- Immunogen
- Recombinant human RNF43-Fc protein
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Type
- Mouse IgG
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Immunohistochemistry; Immunoprecipitation
- Related Disease
- Colorectal cancer,lung cancer.
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-327CT, RRID: AB_3111912)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RNF43"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-328CT | Humanized Anti-RNF43 Recombinant Antibody (TAB-328CT) | WB, ELISA, FC, IHC, IP | Humanized IgG1, κ |
| TAB-328CT-S(P) | Humanized Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-328CT-S(P)) | WB, ELISA, FC, IHC, IP | Humanized scFv |
| TAB-328CT-F(E) | Humanized Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-328CT-F(E)) | WB, ELISA, FC, IHC, IP | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-327CT-S(P) | Mouse Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-327CT-S(P)) | WB, ELISA, FC, IHC, IP | Mouse scFv |
| TAB-327CT-F(E) | Mouse Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-327CT-F(E)) | WB, ELISA, FC, IHC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1185 | PE-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer | FCM, IHC | |
| MHC-CN1186 | APC-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer | FCM, IHC | |
| MHC-YF705 | A*02:01/Human RNF43 (ALWPWLLMA) MHC Pentamer | FCM | |
| MHC-YF706 | A*02:01/Human RNF43 (ALWPWLLMAT) MHC Pentamer | FCM | |
| MHC-YF707 | A*24:02/Human RNF43 (722Y) (NYQPVWLCL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC181 | Recombinant Anti-RNF43 Vesicular Antibody, EV Displayed (VS-0425-YC181) | ELISA, FC, Cell-uptake |
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